Journal: Scientific Reports
Article Title: Proteomics on choroidal neovascularization based on itraq and the protective effect of TAB1 in CNV
doi: 10.1038/s41598-025-15134-1
Figure Lengend Snippet: Verification of the eight types of candidate protein by western blotting (n = 3). ( A , C , E ) The expressions of the candidate differently-expressed proteins, namely MCM7, YES1, SEPT 9, p27 Kip1, p57 Kip2, TRIM 32, RPIA and TAB1. ( B , D , F ) As the statistical result was shown, expressions of TRIM 32, RPIA, and TAB1 were significantly down-regulated while expressions of MCM7, YES1, p27 kip, SEPT 9, and p57 kip 2 did not significantly differ from the control. (Note, ns = no significance; * = P < 0.05; ** = P < 0.01; *** = P < 0.001).
Article Snippet: The primary antibodies used were as followed: Anti-MCM7(abcam, ab52489), anti-YES1(abcam, ab109744), anti-SEPT9(abnova, H00010801-A01), anti-P27 kip1 (CST, #2552), anti-P57 kip2 (Santa cruz, sc-56341), anti-TRIM 32 (Abcam, ab96612), anti-RPIA (Santa Cruz, SC515328), Anti-TAB1 (Santa Cruz, SC166138), HRP-tagged goat anti-rabbit antibody (Bioss, bs-80295G-HRP); HRP tagged goat anti-mouse antibody (bs-40296G-HRP); anti-NF-κBp52 (Abcam,ab129097), anti-RelB(Abcam, ab309084).
Techniques: Western Blot, Control